α-L- 鼠李糖苷酶（α-L-rhamnosidase ,EC 3.2.1.40）是一種水解酶，它主要作用于α-1,2、α-1,3、α-1,4、α-1,6以及α1連接的糖苷鍵，能夠水解結合于底物末端非還原性的α-L- 鼠李糖苷鍵，同時釋放出鼠李糖和相應的配基。

Α-L-rhamnosidase (EC 3.2.1.40) is a hydrolase that acts on α-1,2, α-1,3, α-1.4, α-1,6 and Α-1-linked glycosidic bonds, it can hydrolyze α-L-rhamnoside bonds that bind to the non-reductive end of the substrate and release rhamnose and corresponding ligands.